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采用索氏提取法对何首乌(RPM)和首乌藤(CFPM)的不同部位进行提取,以96微孔板法测定了其对α-葡萄糖苷酶的抑制作用。对何首乌不同溶剂提取物对α-葡萄糖苷酶的抑制作用进行了研究,并首次对首乌藤不同溶剂提取物对α-葡萄糖苷酶抑制作用进行研究。结果发现,何首乌各溶剂提取物均具有较好的α-葡萄糖苷酶抑制作用。何首乌和首乌藤的甲醇、乙酸乙酯和石油醚提取物的IC50值由小到大的顺序为:RPMM(3.83 mg/L)、RPME(28.85 mg/L)、RPMP(62.61 mg/L)、CFPMP(212.42 mg/L)、CFPME(882.14 mg/L)、CFPMM(1 247.80 mg/L),各提取物(除CFPMM)的抑制活性均大于阳性对照Acarbose(1 081.27 mg/L)。研究结果表明,何首乌和首乌藤均具有一定的α-葡萄糖苷酶抑制作用,可以通过体内实验进一步研究两者的降血糖活性,均具有良好的潜在开发价值。该文报道工作的新颖性,已为河南大学图书馆2009年6月24日出具的第CX200906241号《科技查新报告》所证实。
Different parts of Polygonum multiflorum (RPM) and Polygonum multiflorum (CFPM) were extracted by Soxhlet extraction. The inhibitory effect on α-glucosidase was determined by 96 microplate method. Polygonum multiflorum extract of different solvents on α-glucosidase inhibition was studied, and for the first time Polygonum different solvent extract on α-glucosidase inhibition. The results showed that the Polygonum multiflorum Solvent extracts have better α-glucosidase inhibition. The IC50 values of methanol, ethyl acetate and petroleum ether extracts of Polygonum multiflorum and Polygonum multiflorum were RPMM (3.83 mg / L), RPME (28.85 mg / L) and RPMP (62.61 mg / L) , CFPMP (212.42 mg / L), CFPME (882.14 mg / L) and CFPMM (1 247.80 mg / L). The inhibitory activities of all the extracts except CFPMM were higher than that of the positive control Acarbose (1 081.27 mg / L). The results showed that Polygonum multiflorum and Polygonum multiflorum had some inhibitory effects on α-glucosidase, which could be further investigated by in vivo experiments, all of which had good potential value for development. This article reports the novelty of the work and has been confirmed by the Science and Technology Examination Report No. CX200906241 issued by Henan University Library on June 24, 2009.