Objective:To evaluate and-diarrhoeal and antimicrobial activities of the bioactive fraction of Trichilia emetica in order to provide a scientific basis for the management of gastroenteritis in Burkina Faso.Methods:To do this,polyphenols content of extract and fractions were investigated.FolinCiocalteu,AlCl_3 methods and tannic acid respectively were used for polyphenols content.The in vivo antidiarrhocal activity was done using Swiss albino mice of both sexes.In vitro antimicrobial activity(disc-diffusion assay,minimum inhibitory concentration and minimum microbicidal concentration or minimal bactericidal concentration) was assessed using seven bacteria strains(Gram-negative and Gram-positive).Results:About our study,it was found that ethyl acetate fraction effective attenuation factior(EAF) elicits the higher total phenolics and total flavonoids contents compared to the extracts of leaves of Trichilici emetica.EAF of Trichilia emetica Vahl..has positive effects in a dose dependent manner against diarrhoea induced by castor oil in experimental mice.The bioactive fraction also showed good antimicrobial activity against all tested Gram-negative and Grampositive bacteria strains.It was shown that experimental bacteria strains were more sensitive to the EAF effect compared to the ciprofloxacin.Conclusions:The obtained results allow justifying the traditional uses of Trichilia emetica and possess good antidiarrhoeal and antimicrobial activities of EAF from Trichilia emetica.Results of the present study have clearly supported the utilization of Trichilia emetica in Burkina Faso traditional medicine. Objective: To evaluate and-diarrhea and antimicrobial activities of the bioactive fraction of Trichilia emetica in order to provide a scientific basis for the management of gastroenteritis in Burkina Faso. Methods: To do this, polyphenols content of extract and fractions were investigated. FolinCiocalteu, AlCl 3 methods and tannic acid were respectively used for polyphenols content. In vivo antidiarrh local activity was done using Swiss albino mice of both sexes. In vitro antimicrobial activity (disc-diffusion assay, minimum inhibitory concentration and minimal microbicidal concentration or minimal bactericidal concentration) was assessed using seven bacteria Gram-negative and Gram-positive. Results: About our study, it was found that ethyl acetate fraction effective attenuation factior (EAF) elicits the higher total phenolics and total flavonoids contents compared to the extracts of leaves of Trichilici emetica. EAF of Trichilia emetica Vahl..has positive effects in a dose dependent man ner against diarrhea induced by castor oil in experimental mice. The bioactive fraction also showed good antimicrobial activity against all tested Gram-negative and Gram-positive bacteria strains. It was shown that the experimental bacteria strain was more sensitive to the EAF effect compared to the ciprofloxacin. Conclusions : The obtained results allow justifying the traditional uses of Trichilia emetica and possess good antidiarrhoeal and antimicrobial activities of EAF from Trichilia emetica. Results of the present study have clearly supported the utilization of Trichilia emetica in Burkina Faso traditional medicine.