神经干细胞(Neural stem cell,NSC)移植后,如何监测其在宿主体内的存活、迁移以及与宿主组织的整合情况一直是NSC移植中的一项关键技术难题。传统的方法是采用荧光染料标记并行免疫组化切片分析鉴定,不能动态观测移植NSC的生化、分化情况。近年来,超顺氧化铁、放射性核素、荧光蛋白等开始用于NSC移植示踪,实现了移植后的NSC在活体内无创性地动态监测。 How to monitor its survival, migration and integration with host tissues after transplantation of neural stem cells has been a key technical challenge in NSC transplantation. The traditional method is the use of fluorescent dye labeling parallel immunohistochemical slice analysis identification, can not dynamically observe the biochemical and differentiation of transplanted NSC. In recent years, super-ferric oxide, radionuclides, fluorescent proteins began to be used for NSC transplants tracing, to achieve a non-invasive dynamic monitoring of the transplanted NSCs in vivo.