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原理:当食物变应原和吸附在小肠固有层的肥大细胞上的IgE接触时,释放组织胺等介质,导致小肠渗透性的增加,这时巨分子能穿越小肠屏障。本法测定在口服甘露醇(一种小分子,在正常情况下能穿越小肠屏障)和乳果糖(一种大分子,在正常情况下不能穿越小肠屏障)后尿内二者的排泄量。实验方法:受试者从隔夜起禁食,次日清晨饮含有甘露酵及乳果糖各5g的65ml糖水,饮后前2
Principle: When food allergens and IgE adsorbed on mast cells in the lamina propria of the small intestine contact release of histamine and other mediators, leading to increased permeability of the small intestine, when giant molecules can cross the intestinal barrier. This method measures the excretion of both mannitol in oral mannitol (a small molecule that crosses the intestinal barrier under normal conditions) and lactulose (a macromolecule that normally can not cross the intestinal barrier). Experimental methods: subjects from fasting overnight, drink the morning of the next morning with 5g of mannan and lactulose each of the 65ml of sugar, 2