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采用本实验室构建的丝状蓝藻Calothrixsp.PCC7601基因整合平台系统供体质粒pUTK转化单胞蓝藻Synechococcussp.PCC7942,通过抗性筛选获得了具卡那霉素抗性的转化藻株。经Southern杂交证实 pUTK部分序列已整合到Synechococcussp.7942染色体DNA上 ;红光诱导后通过蛋白质SDS PAGE电泳 ,Westernblot证实 pUTK的胸腺素α1 基因得以有效表达,表达量达到总蛋白的4.8 %。结果表明,基因整合平台系统供体质粒 pUTK不仅可转化丝状蓝藻Calothrixsp.PCC7601,还可转化单胞蓝藻Synechococcussp.PCC7942。
The constructed cyanobacteria Calothrix sp. PCC7601 gene integration platform system donor plasmid pUTK transformed monocytogenes Synechococcussp. PCC7942, kanamycin-resistant transformed algal strains obtained by resistance screening. Southern blotting confirmed that the partial sequence of pUTK was integrated into the chromosome DNA of Synechococcussp.7942. After induced by red light, SDS-PAGE electrophoresis showed that the expression of pUTK was up to 4.8% of the total protein. The results showed that the donor plasmid pUTK of the platform could transform not only Calothrix sp. PCC7601, but also Synechococcus sp. PCC7942.