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目的:研究双氢青蒿素对人胰腺癌细胞株SW-1990增殖及凋亡的影响,并探讨其作用机制。方法:采用细胞增殖/毒性检测试剂盒(Cell Counting Kit-8,CCK-8)检测不同浓度双氢青蒿素(dihydroartemisinin,DHA)对人胰腺癌细胞株SW-1990增殖的影响;流式细胞术Annexin V-FITC和PI双标染色法检测不同浓度DHA对其凋亡的影响;并且用激光共聚焦显微镜观察在Hoechst 33342/PI荧光双染色下胰腺癌细胞SW-1990形态学变化;RT-PCR检测DHA对胰腺癌细胞SW-1990中端粒酶催化亚单位hTERT mRNA表达的影响。结果:DHA在体外可以明显的抑制SW-1990的增殖,并且具有浓度-时间依赖性;DHA在体外能明显诱导SW-1990细胞的凋亡;DHA可以抑制胰腺癌细胞SW-1990中hTERT mRNA的表达,并且与浓度呈正相关。结论:DHA在体外抑制胰人腺癌细胞SW-1990的增殖、诱导其凋亡,其作用机制可能是抑制了肿瘤细胞中端粒酶催化亚单位hTERT mRNA的表达。
Objective: To study the effect of dihydroartemisinin on the proliferation and apoptosis of human pancreatic cancer cell line SW-1990 and to explore its mechanism. Methods: Cell Counting Kit-8 (CCK-8) was used to detect the effect of dihydroartemisinin (DHA) on the proliferation of human pancreatic cancer cell line SW-1990. Flow cytometry Annexin V-FITC and PI double staining were used to detect the apoptotic effect of DHA at different concentrations. The morphological changes of SW-1990 pancreatic cancer cells were observed by laser confocal microscopy with double staining of Hoechst 33342 / PCR to detect the effect of DHA on telomerase catalytic subunit hTERT mRNA expression in SW-1990 pancreatic cancer cells. RESULTS: DHA inhibited the proliferation of SW-1990 in vitro in a time-and concentration-dependent manner. DHA significantly induced apoptosis in SW-1990 cells in vitro. DHA inhibited the expression of hTERT mRNA in SW-1990 cells Expression, and with the concentration was positively correlated. Conclusion: DHA inhibits the proliferation and induces apoptosis of pancreatic adenocarcinoma cell line SW-1990 in vitro. The possible mechanism is that DHA inhibits the expression of telomerase catalytic subunit hTERT mRNA in tumor cells.