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玉米大斑病菌（Ｅｘｓｅｒｏｈｉｌｕｍ ｔｕｒｃｉｃｕｍ）在改良Ｆｒｉｅｓ培养液中培养可以产生ＨＴ－毒素。 ＴＬＣ分析表明： １号小种毒素至少有２种毒性成分，２号小种毒素至少有３种毒性成分。通过ＧＣ－ＭＳ、ＨＲＭＳ、ＮＭＲ分析表明组分１的分子式为Ｃ６Ｈ６Ｏ３，分子量１２６．０３２８，其化学结构为５－羟甲基－２－呋喃甲醛。组分Ⅱ分子量为１２４，初步推测为２，５－二甲醛呋喃。毒素钝化试验表明，代森锰锌、Ｎａ２ＭｏＯ４、ＮａＮＯ３、ＺｎＳＯ４、ＫＭｎＯ４、ＫＩ及４种糖类可钝化毒素使其毒性降低；而ＣｕＳＯ４、ＮａＣＩ、ＫＣｒ２Ｏ５、Ｈ２Ｏ２则可使毒素毒性增强。毒素处理玉米叶片后，细胞膜透性、细胞的ＣＡＴ、Ｖｃ氧化酶、ＳＯＤ、ＰＯＤ、ＰＡＬ、ＰＰＯ活性都发生了一定规律变化，而毒素中加入Ｈ２Ｏ２和代森锰锌可抑制或加强这种变化。
Exserohilum turcicum is cultured in modified Fries medium to produce HT-toxin. TLC analysis showed that no. 1 toxin had at least 2 toxic components and no. 2 toxin had at least 3 toxic components. Analysis by GC-MS, HRMS and NMR indicated that component 1 has the molecular formula of C6H6O3 and the molecular weight of 126.0328. Its chemical structure is 5-hydroxymethyl-2-furaldehyde. Component II molecular weight of 124, initially speculated that 2,5-dimethylformaldehyde furan. The toxin passivation test showed that the toxicity of mancozeb, Na2MoO4, NaNO3, ZnSO4, KMnO4, KI and four kinds of sugars can be detoxified to reduce the toxicity; and CuSO4, NaCI, KCr2O5, H2O2 can make toxin toxicity increased. Toxin treatment of corn leaves, cell membrane permeability, cell activity of CAT, Vc oxidase, SOD, POD, PAL, PPO have undergone some changes in the law, and the addition of H2O2 and mancozeb toxin can inhibit or enhance the change .