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目的:观察复智胶囊主要成分大黄素对体外培养的SH-SY5Y细胞延迟整流钾电流(IK)的影响。方法:体外培养SH-SY5Y,MTT法检测大黄素细胞毒性确定加药浓度,将SH-SY5Y分为空白对照组、OGD组、糖氧剥夺OGD+大黄素(Xμmol/L)治疗组,全细胞膜片钳技术描记IK变化并绘制I-V曲线。结果:SH-SY5Y经过糖氧剥夺后细胞存活率显著下降,IK电流密度减弱,此现象可被大黄素逆转,在指令电压为+50mV时IK峰密度值分别从(4.474±0.4182)pA/pF增大至(5.947±0.7415)pA/pF(P<0.05,n=15);大黄素可使被OGD抑制的IK电流-电压曲线(I-V)慢慢上升,并且伴随着的去极化幅度的逐步升高而越来越大。结论:大黄素对SH-SY5Y细胞具有保护作用;对IK有激活作用。
Objective: To observe the effect of emodin, the main component of Fuzhi Capsule, on the delayed rectifier potassium current (IK) in cultured SH-SY5Y cells. Methods: SH-SY5Y cells were cultured in vitro. The cytotoxicity of emodin was assayed by MTT assay. The SH-SY5Y cells were divided into blank control group, OGD group, OGD + emodin (Xμmol / L) Clamp technique to characterize IK changes and plot IV curves. RESULTS: The viability of SH-SY5Y cells was significantly decreased after glucose-oxygen deprivation, and the IK current density was weakened. This phenomenon could be reversed by emodin. The IK peak densities at (+4) mV and (+4.4) pA / (5.947 ± 0.7415) pA / pF (P <0.05, n = 15). Emodin could slowly increase the IK current-voltage curve (IV) suppressed by OGD with the accompanying amplitude of depolarization Gradually rising and growing. Conclusion: Emodin can protect SH-SY5Y cells and activate IK.