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本文观察10个孕24～28天的白色澳洲家兔,在子宫胎盘缺血后,子宫和肾释放肾素的情况。其结果说明正常孕兔的肾是肾素主要来源,但在急性子宫胎盘局部缺血时即使肾脏完整,子宫也可释放肾素。本研究选用的孕兔体重3.5～4.5kg(41±0.4)。均用戊巴比妥30 mg/kg体重静脉麻醉,将聚乙烯管插入股动脉并接到三通上,用转移器监护血压脉搏,开腹直接从子宫动、静脉及右侧肾静脉采血,标本立即按规定保存备用取血后,结扎子宫动脉。结扎后30和60分钟分别再采血,用放免方法测定肾素活性,用血管紧张素Ⅰ免疫药箱(Squibb)pH5.5,数据用t试验统计处理,所有结果用平均数±SE。
This article observed 10 pregnant 24 to 28 days of white Australian rabbit, uterine and renal renin release after uterine placental ischemia. The results show that normal pregnant rabbit kidney is the main source of renin, but in the acute uterine placental ischemia, even if the kidney is intact, the uterus can release renin. The pregnant rabbits used in this study weighed 3.5-4.5 kg (41 ± 0.4). Both were intravenously anesthetized with pentobarbital 30 mg / kg body weight. The polyethylene tube was inserted into the femoral artery and connected to the tee. The blood pressure pulse was monitored by a transfer device. Blood was collected directly from the uterine artery, vein and right renal vein, Specimens immediately stored in reserve according to the provisions of blood, the uterine artery ligation. Blood was collected 30 and 60 minutes after ligation, and renin activity was measured by radioimmunoassay. Squibb pH 5.5 was used. The data were statistically analyzed using the t test. All results were expressed as means ± SE.