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目的建立转染卵泡刺激素受体(FSHR)307和680位点突变的上皮性卵巢癌SKOV3细胞系和裸鼠动物模型。方法将人卵巢颗粒细胞(来自体外受精取卵时废弃的颗粒细胞),行原代培养后用于FSHR RNA提取;FSHR和FSHR307、680位点突变重组蛋白构建;将FSHR和FSHR307、680位点突变蛋白转染SKOV3细胞株;将处理后的3组SKOV3细胞接种到裸鼠皮下,8周后处死裸鼠,并取瘤组织验证。结果建立了307和680位点突变的FSH受体高表达的上皮性卵巢癌SKOV3细胞系和裸鼠动物模型。结论成功地建立了307和680位点突变的FSH受体高表达的SKOV3上皮性卵巢癌细胞系和裸鼠移植瘤模型,为FSH在上皮性卵巢癌中的作用和治疗提供了一种新的细胞模型和动物模型。
Objective To establish an epithelial ovarian cancer SKOV3 cell line transfected with FSHR at 307 and 680 loci and a nude mouse model. Methods Human ovarian granulosa cells (exfoliated granulosa cells from in vitro fertilization) were cultured and used for FSHR RNA extraction. FSHR and FSHR307,680 mutants were constructed. The FSHR and FSHR 307,680 loci The mutant protein was transfected into SKOV3 cell line. Three SKOV3 cells were inoculated subcutaneously into nude mice. After 8 weeks, the nude mice were sacrificed and tumor tissue was taken for verification. Results The ovarian cancer SKOV3 cell lines and nude mice models with high FSH receptor expression at 307 and 680 loci were established. Conclusion The SKOV3 epithelial ovarian cancer cell line and nude mouse xenograft model with FSH receptor highly expressed in 307 and 680 loci have been established successfully, which provides a new method for the role and treatment of FSH in epithelial ovarian cancer Cell model and animal model.