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应用补体结合血清学反应和萤光标记抗体间接染色法研究了大鼠肝器官特异性抗原和在移植瘤中的变化。发现肝粒线体中存在着器官特异性抗原,经萤光标记抗体测定,可以看出这种器官特异性抗原是均匀地分布在肝实质细胞的细胞质中的,未能证实Nairn等所看到细胞膜的特强萤光的结果,这种差别可能是由于我们所用的抗原和Nairn等所用的抗原不同之故。当肝组织变成肝癌后,这一种器官特异性抗原消失了。应用直接染色法时,我们未能证实Weiler的结果。
Complement-binding serological reaction and fluorescent-labeled antibody indirect staining were used to study the changes of rat liver-specific antigen and its transplanted tumor. The presence of organ-specific antigens in the liver mitochondria, as measured by fluorescently labeled antibodies, shows that this organ-specific antigen is evenly distributed in the cytoplasm of hepatic parenchymal cells and failed to confirm what Nairn et al. The distinctive fluorescence of the cell membrane may be due to the difference between the antigens we used and those used by Nairn et al. When liver tissue becomes liver cancer, this organ-specific antigen disappears. We failed to confirm Weiler’s results when applying direct staining.