论文部分内容阅读
从我国不同地区、不同松树上采集代表ＣｒｏｎａｒｔｉｕｍｒｉｂｉｃｏｌａＣｆｌａｃｃｉｄｕｍ及Ｃ。ｑｕｅｒｃｕｕｍ３种松干锈菌的５８个菌株，分析了其种间及种内的同工酶变异。电泳检测过的１３种酶中有８种显出酶带，其中酯酶（ＥＳＴ）同工酶谱具最显著而稳定的种间差异，可作为区别鉴定３种松干锈菌的有效手段。在来自东北各地红松上的１６个Ｃ．ｒｉｂｉｃｏｌａ菌株中未发现任何酶谱变异，显示了本菌群体遗传性质的均一。来自东北地区樟子松上的１７个Ｃｆｌａｃｃｉｄｕｍ菌株中存在酶谱变异，但多数菌株的酶谱同来自安徽及陕西马尾松上的菌株完全一致，此结果支持把我国的Ｃｆｌａｃｃｉｄｕｍ看成一个集合种。来自我国南北不同地区不同松树的Ｃｑｕｅｒｃｕｕｍ菌株，除樟子松上的部分菌株外，其酶谱完全相同，尚未分化出北美的那种专化型间的酶谱差异，可能暗示我国松瘤锈菌的起源晚于北美。
From different parts of China, different pine on behalf of CronartiumribicolaCflaccidum and C. Fifty-eight strains of quercuum, Tricholoma matsutake and Tricholoma matsutake were analyzed for isozyme variation within and between species. Among the 13 enzymes detected by electrophoresis, 8 showed enzyme bands, among which Esterase (EST) isozymes had the most significant and stable interspecific differences, which could be used as an effective method to distinguish and identify 3 species of Puccinia recondita. 16 C on the pine from all over Northeast China. No zymogram variations were found in the ribicola strains, indicating a homogenous genetic nature of this bacterial population. There were zymograms in 17 Cflaccidum strains from Pinus sylvestris var. Mongolica in northeastern China. However, the enzyme patterns of most strains were identical with those from Masson pine from Anhui and Shaanxi Provinces. This result supported the identification of Cflaccidum in China as a species pool. Cquercuum strains from different pine trees in different regions of China, except for some strains on Pinus sylvestris, had the same zymogram and no difference in the zymogram of the specialized type that had not been differentiated from North America, The origin of later than North America.