论文部分内容阅读
目的 探讨延髓内脏带 (MVZ)与下丘脑室旁核 (PVN)和视上核 (SON)之间是否存在往返渗透压投射通路。方法 采用给大鼠饮用 3%氯化钠的方法复制高渗刺激模型 ,并用WGA HRP逆行追踪、抗Fos和抗酪氨酸羟化酶 (TH)或加压素 (VP)及胶质原纤维酸性蛋白 (GFAP)免疫组织化学相结合的四重标记方法 ,观察MVZ、PVN和SON中WGA HRP、Fos、TH、VP和GFAP的阳性分布及表达状况。结果 高渗刺激后MVZ、PVN和SON内Fos阳性细胞明显增多。GFAP阳性结构也明显增多 ,其分布与Fos阳性细胞分布基本一致 ,表现为胞体肥大、突起粗长。AST紧密包绕在神经元周围形成神经元 星形胶质细胞 (AST)复合体 (N ASC)。结论 神经元和AST以N ASC的形式共同参与渗透压调节反应 ,体内存在MVZ和SON或PVN之间往返的渗透压调节通路。
Objective To investigate whether there is a reciprocating osmotic pressure projection pathway between medullary visceral zone (MVZ) and hypothalamic paraventricular nucleus (PVN) and supraoptic nucleus (SON). Methods Hypertonic stimulation model was reproduced by drinking 3% sodium chloride in rats. WGA HRP retrograde tracing, anti-Fos and anti-tyrosine hydroxylase (TH) or vasopressin (VP) and glial fibrils (GFAP) immunohistochemistry was used to observe the positive distribution and expression of WGA HRP, Fos, TH, VP and GFAP in MVZ, PVN and SON. Results After hypertonic stimulation, Fos positive cells in MVZ, PVN and SON were significantly increased. GFAP positive structure was also significantly increased, the distribution and distribution of Fos-positive cells are basically the same, manifested as cell body hypertrophy, protuberances coarse. The AST tightly wraps around the neurons to form a neuronal astrocyte (AST) complex (N ASC). Conclusion The neurons and AST participate in the osmoregulation reaction in the form of N ASC, and there is an osmotic pressure regulating pathway between MVZ and SON or PVN in vivo.