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目的建立以聚酰胺薄膜为固定相,微乳液为展开剂,同时鉴别2种洋甘菊中5种化合物木犀草素、芹菜素、木犀草素-7-葡萄糖苷、芹苷元-7-葡萄糖苷、7-甲氧基香豆素的方法。方法以SDS-正丁醇-正庚烷-水微乳液为展开剂,并配以甲酸为有机改性剂,以2-氨基乙基联苯基硼酸酯、聚乙二醇400甲醇溶液为显色剂,考察了不同微乳液对两种洋甘菊甲醇提取物分离度的影响,通过调节展开剂的组成,寻找出最佳的薄层分离条件并分离鉴别了母菊、罗马洋甘菊。结果微乳液-甲酸(9∶2)为适宜的展开剂,其中微乳液SDS-正丁醇-正庚烷质量比为0.27∶0.63∶0.10、含水量75%。与硅胶薄层比较,分离效果明显提高。结论建立了一种分离鉴别母菊、罗马洋甘菊中木犀草素、芹菜素、木犀草素-7-葡萄糖苷、芹甙元-7-葡萄糖苷、7-甲氧基香豆素5种化合物的方法。方法简便、快速和准确。
OBJECTIVE To establish a polyimides film as stationary phase and microemulsion as developing solvent, and identify five compounds, luteolin, apigenin, luteolin-7-glucoside, cephalan-7-glucoside, 7 - methoxy coumarin method. Methods With SDS-n-butanol-n-heptane-water microemulsion as developing solvent and formic acid as organic modifier, 2-aminoethylbiphenyl borate and polyethylene glycol 400 methanol solution were The effect of different microemulsion on the separation of methanol extract from two kinds of chamomile was investigated. The optimum conditions of thin layer separation were determined by adjusting the composition of the solvent. And the chamomile and chamomile were separated and identified. Results Microemulsion-formic acid (9: 2) was the suitable developing solvent. The mass ratio of SDS-n-butanol to n-heptane in the microemulsion was 0.27: 0.63: 0.10 and the water content was 75%. Compared with the thin layer of silica gel, the separation effect is obviously improved. Conclusion A new method for the isolation and identification of five compounds of matricaria, Roman chamomile, luteolin, apigenin, luteolin-7-glucoside, carbendazim-7-glucoside and 7-methoxycoumarin method. The method is simple, fast and accurate.