论文部分内容阅读
Objective To observe the effects of high glucose and anoxia on Amot expression in vascular endothelial cells(VECs),and explore its role in angiogenesis.Methods VECs were incubated with different glucose concentrations for 48 h,and then cultured at normal oxygen concentration or anaerobic condition for 24 h.The protein expressions of p130-Amot and p80-Amot were detected by Western blot.After Amot expression was downregulated in VECs by siRNA,wound healing experiments and angiogenesis experiments were performed to test the effect
Objective To observe the effects of high glucose and anoxia on Amot expression in vascular endothelial cells (VECs), and explore its role in angiogenesis. Methods VECs were incubated with different glucose concentrations for 48 h, and then cultured at normal oxygen concentration or anaerobic condition for 24 h. The protein expressions of p130-Amot and p80-Amot were detected by Western blot. After Amot expression was downregulated in VECs by siRNA, wound healing experiments and angiogenesis experiments were performed to test the effect