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目的:通过对广东、广西2个产地多批次、多规格桂枝中Fe、Mg等24种微量元素的测定及形态分析,探索桂枝微量元素的量变规律及形态特征。方法:按照传统煎煮法对桂枝中24种元素进行提取;用0.45μm微孔滤膜分离提取液中的可溶态与悬浮态微量元素;用LSA-10大孔吸附树脂柱分离可溶态中的有机态与无机态微量元素;并采用正辛醇/水分配体系,在模拟人体胃肠环境条件下,将可溶态中这24种元素分离为醇溶态和水溶态;采用微波消解-电感耦合等离子体质谱法(ICP-MS法)测定桂枝原药、水煎液及水煎液各种形态中24种微量元素的含量;采用SPSS 19.0软件对微量元素进行相关分析。结果:24种微量元素的线性关系系数(r)均大于0.999 0,原药及提取液中元素测定方法的精密度、稳定性和回收率均能满足实验要求,不同产地、不同规格桂枝中部分微量元素含量有显著差异(P<0.05),桂枝中微量元素之间存在显著的或极显著的正相关或负相关(P<0.05)。微量元素的提取率为1.0%~59.4%,可溶态在水中的比率为61.8%~99.1%,悬浮态比率为38.2%~0.9%,微量元素在模拟的人体胃肠环境中的醇溶态溶出率在2%以下,微量元素主要以无机态和水溶态的形式存在。结论:不同微量元素提取率差异显著,可溶态微量元素含量高于悬浮态微量元素的含量,人体胃肠环境醇溶态含量差异不大,小于水溶态的含量,微量元素之间的相关性及形态分析对桂枝临床合理应用具有指导意义。
OBJECTIVE: To investigate the quantitative changes and morphological characteristics of trace elements in Cassia twig, through the determination and speciation analysis of 24 trace elements such as Fe and Mg in Guiyi and Guiyi, two producing areas in Guangdong and Guangxi. Methods: 24 kinds of elements in Guizhi were extracted by traditional decoction method. The soluble and suspended trace elements in the extract were separated by 0.45μm microporous membrane. The fraction was soluble with LSA-10 macroporous resin. State organic and inorganic trace elements; and n-octanol / water partitioning system, in simulated human gastrointestinal environment, the soluble elements of these 24 elements was separated into alcohol-soluble and water-soluble; using microwave Determination of 24 trace elements in various forms of Guizhi drug, decoction and decoction by inductively coupled plasma mass spectrometry (ICP-MS); Correlation analysis of trace elements by SPSS 19.0 software. Results: The linear relationship coefficients (r) of 24 kinds of trace elements were all greater than 0.999 0. The precision, stability and recovery of elemental determination method of the original drug and the extract could meet the experimental requirements. (P <0.05). There was a significant or highly significant positive or negative correlation between trace elements in Guizhi (P <0.05). The extraction rates of trace elements ranged from 1.0% to 59.4%, the ratios of soluble to water in water ranged from 61.8% to 99.1%, and the suspended ratios ranged from 38.2% to 0.9%. The contents of trace elements in simulated human gastrointestinal environment Dissolution rate of 2% or less, trace elements mainly in the form of inorganic and water-soluble form. CONCLUSION: The extraction rates of different trace elements are significantly different. The content of soluble trace elements is higher than that of suspended trace elements. There is no significant difference in the content of alcohol in human gastrointestinal environment, less than the content of water soluble and the correlation between trace elements And morphological analysis Gui Zhi clinical application is instructive.