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目的探讨视黄醇结合蛋白(RBP)4在慢性阻塞性肺疾病(慢阻肺)血清中的表达及其和炎症反应、氧化应激的关系。方法用酶联免疫吸附法(ELISA)测定四川大学华西医院2014年6—12月97例慢阻肺患者和47例对照者血清RBP4浓度,应用液相芯片技术测定两组C反应蛋白(CRP)、髓过氧化物酶(MPO)浓度,并测定肺功能指标。分析RBP4浓度在两组的差异,观察其与CRP、MPO、肺功能指标的关系。结果慢阻肺组血清RPP4表达明显高于对照组[(78.33±30.13)mg/L对(51.55±17.92)mg/L,P<0.05]。RBP4的表达与CRP的表达正相关(r=0.432,P<0.001),与MPO的表达正相关(r=0.338,P<0.001),与第一秒用力呼气容积占预计值百分比(FEV1%)呈负相关(r=-0.263,P=0.001),与FEV1占用力肺活量(FVC)百分比呈负相关(r=-0.339,P<0.001)。结论慢阻肺患者血清RBP4表达和肺功能有密切关系。氧化应激、炎症反应可能会导致RBP4表达上升。
Objective To investigate the expression of retinol binding protein 4 (RBP) 4 in serum of chronic obstructive pulmonary disease (COPD) and its relationship with inflammation and oxidative stress. Methods Serum RBP4 levels in 97 patients with chronic obstructive pulmonary disease (COPD) and 47 controls at West China Hospital of Sichuan University from June to December in 2014 were measured by enzyme-linked immunosorbent assay (ELISA). The levels of C-reactive protein (CRP) , Myeloperoxidase (MPO) concentration, and determination of lung function. The difference of RBP4 concentration between the two groups was analyzed, and the relationship between them and CRP, MPO and pulmonary function indexes was observed. Results The expression of RPP4 in COPD group was significantly higher than that in control group [(78.33 ± 30.13) mg / L vs (51.55 ± 17.92) mg / L, P <0.05]. The expression of RBP4 was positively correlated with the expression of CRP (r = 0.432, P <0.001) and positively correlated with the expression of MPO (r = 0.338, P <0.001) ) (R = -0.263, P = 0.001), and negatively correlated with the percentage of FEV1 occupancy FVC (r = -0.339, P <0.001). Conclusion Serum RBP4 expression in COPD patients is closely related to pulmonary function. Oxidative stress, inflammatory response may lead to increased RBP4 expression.