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目的：观察家兔病理性氧供依赖性（ｐａｔｈｏｌｏｇｉｃａｌｏｘｙｇｅｎｓｕｐｐｌｙｄｅｐｅｎｄｅｎｃｅ，ＰＯＳＤ）模型中各组织，特别是牙髓组织的病理学改变，以及抗ｒｈＴＮＦ单克隆抗体对实验动物是否有保护作用。方法：２８只大耳白兔随机分为对照、内毒素、抗体保护和无关抗体４组，每组７只。以小剂量大肠杆菌内毒素分次注射复制ＰＯＳＤ模型。ＥＬＩＳＡ测定血中ＴＮＦ浓度。采用组织学、组织化学和免疫组化法评价牙髓形态学特征。结果：①内毒素组与无关抗体组的牙髓组织有充血、水肿、渗出、弥漫性血管内凝血等病理变化，而对照组和抗体保护组形态正常；②内毒素组的ＴＮＦ浓度明显高于对照组（Ｐ＜０．０１），抗体保护组的ＴＮＦ浓度则明显低于无关抗体组（Ｐ＜０．０１）。结论：内毒素血症可致牙髓组织的病理学改变，抗ｒｈＴＮＦ单抗可以阻断其病变，具有临床应用前景。
OBJECTIVE: To observe the pathological changes of tissues, especially dental pulp in pathological oxygen donor dependability (POSD) model and whether the anti-rhTNF monoclonal antibody has a protective effect on experimental animals. Methods: Twenty-eight large white rabbits were randomly divided into 4 groups: control, endotoxin, antibody protection and irrelevant antibody, 7 in each group. A small dose of E. coli endotoxin injected subcutaneously to duplicate the POSD model. ELISA to measure the concentration of TNF in blood. Histological, histochemical and immunohistochemical methods were used to evaluate the dental pulp morphology. Results: (1) Pulp tissue of endotoxin group and irrelevant antibody group had congestion, edema, exudation, diffuse intravascular coagulation and other pathological changes, while the control group and antibody protection group had normal morphology; (2) TNF concentration in endotoxin group was significantly higher In the control group (P <0.01), the concentration of TNF in the antibody-protected group was significantly lower than that in the non-related antibody group (P <0.01). Conclusion: Endotoxemia can cause histopathological changes in pulp tissue. Anti-rhTNF monoclonal antibody can block its pathological changes and has clinical application prospect.