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目的 :探讨染氟大鼠血清中一氧化氮 ( NO)浓度、一氧化氮合酶 ( NOS)活性及超氧化物歧化酶 ( SOD)含量的变化。方法 :血清 NO浓度采用硝酸还原酶法、血清中 NOS活性采用 NOS催化 L—Arg生成 NO法、血清中 SOD含量采用亚硝酸盐显色法。结果 :( 1 )染氟大鼠血清中 NO浓度为 ( 1 1 .1 5± 3.58μ mol/L)、NOS活性为 ( 4.60± 1 .0 7U/ml)均明显低于对照组 NO( 1 5.86± 5.82 μ mol/L)和 NOS( 8.1 1± 1 .2 2 u/ml) ( P<0 .0 5)。 ( 2 )染氟大鼠血清 SOD含量( 84 .53± 8.36NU /ml)与对照组 SOD ( 85.84± 1 4 .4 7NU/ml)差异无显著性 ( P>0 .0 5)。结论 :本文提示染氟可降低体内 NO浓度和 NOS的活性 ,而 NO与 SOD含量之间的关系仍需进一步研究
Objective: To investigate the changes of nitric oxide (NO), nitric oxide synthase (NOS) and superoxide dismutase (SOD) in serum of rats exposed to fluorine. Methods: Nitric acid reductase was used to determine the concentration of NO in serum, NOS was used to catalyze L-Arg to produce NO in serum, and the content of SOD in serum was determined by nitrite method. Results: (1) The concentration of nitric oxide (NO) in the serum of rats stained with fluorine was (11.51 ± 3.58μ mol / L) and the activity of NOS was (4.60 ± 1.07U / ml) 5.86 ± 5.82 μ mol / L) and NOS (8.1 1 ± 1.22 u / ml) (P <0.05). (2) There was no significant difference in serum SOD level between fluorinated rats (84.53 ± 8.36 NU / ml) and control group (85.84 ± 14.7 NU / ml) (P> 0.05). CONCLUSIONS: This paper suggests that fluoride exposure may reduce NO and NOS activity in vivo, but the relationship between NO and SOD still needs further study