论文部分内容阅读
目的探讨子宫内膜癌组织中多药耐药基因（ＭＤＲ１）和多药耐药相关蛋白（ＭＲＰ）基因的表达及其意义。②方法利用半定量逆转录聚合酶链反应（ＲＴ－ＰＣＲ），对３７例初治子宫内膜癌及１０例正常子宫内膜组织ＭＤＲ１和ＭＲＰ基因的表达进行检测。③结果３７例子宫内膜癌和１０例正常子宫内膜组织均有ＭＤＲ１和ＭＲＰ基因的表达，两种基因在这两种组织中的表达水平无显著性差异（ｔ＝１．１７，１．５１，Ｐ＞０．０５）。有７例子宫内膜癌ＭＲＰ基因呈高水平表达（１．６８±０．１７），明显高于其他３０例病人（０．８３±０．１１），差异有极显著性（ｔ＝１６．５５，Ｐ＜０．００１）。④结论子宫内膜癌对化疗的耐药可能是由于固有性多药耐药所致，与ＭＤＲ１和ＭＲＰ基因的表达有关，ＭＲＰ基因的过度表达有可能作为判断子宫内膜癌病人预后的一个指标
Objective To investigate the expression and significance of multidrug resistance gene (MDR1) and multidrug resistance associated protein (MRP) in endometrial carcinoma. Methods Semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expression of MDR1 and MRP genes in 37 cases of untreated endometrial carcinoma and 10 cases of normal endometrium. 3 Results The expression of MDR1 and MRP genes was detected in 37 cases of endometrial carcinoma and 10 cases of normal endometrial tissue. There was no significant difference in the expression of the two genes in these two tissues (t=1.17, 1. 51, P>0.05). In 7 cases of endometrial cancer, MRP gene was highly expressed (1.68±0.17), which was significantly higher than other 30 patients (0.83±0.11). The difference was extremely significant (t=16. 55, P<0.001). 4 Conclusions Resistance to chemotherapy in endometrial cancer may be due to intrinsic multidrug resistance, which is related to the expression of MDR1 and MRP genes. Overexpression of MRP gene may be used as an indicator to determine the prognosis of endometrial cancer patients.