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目的:建立HPLC方法测定血浆去甲万古霉素和万古霉素浓度,并应用于治疗药物监测。方法:采用Zorbax SB C_(18)色谱柱,采用梯度流动相.流动相组成甲醇:pH7.3磷酸缓冲盐水溶液,检测波长230 nm,流速1.0 ml·min~(-1),柱温32℃。采用高氯酸和二氯甲烷去蛋白的方法处理样品。结果:去甲万古霉素和万古霉素与血浆中其他成分分离良好,在2.5～100μg·ml~(-1)范围内线性关系良好,低、中、高3个浓度的绝对回收率去甲万古霉素分别为79.57%,76.46%,84.88%,万古霉素分别为86.52%.87.05%.83.47%。两者的日内、日间RSD均<7%。血浆样品室温放置4 h,冷冻解融2次,处理好的样品室温放置24 h均稳定。结论:该方法灵敏、准确、快速,可用于去甲万古霉素和万古霉素人体血药浓度监测和药物动力学研究。
Objective: To establish a HPLC method for the determination of plasma levels of norvancomycin and vancomycin and to monitor the therapeutic drug. METHODS: Zorbax SB C 18 column was used with gradient mobile phase consisting of methanol: pH 7.3 phosphate buffered saline, detection wavelength 230 nm, flow rate 1.0 ml · min -1, column temperature 32 ℃ . Samples were treated by perchloric acid and methylene chloride deproteinization. Results: Norvancomycin and vancomycin were well separated from other components in plasma. The linear relationship was good in the range of 2.5-100 μg · ml -1, and the absolute recoveries of low, medium and high concentrations were Vancomycin was 79.57%, 76.46%, 84.88%, vancomycin was 86.52% .87.05% .83.47% respectively. Both the day and day RSD were <7%. Plasma samples were placed at room temperature for 4 h, thawed and thawed twice, and the treated samples were stable at room temperature for 24 h. Conclusion: The method is sensitive, accurate and rapid and can be used for the monitoring of human plasma concentration of norvancomycin and vancomycin and pharmacokinetics.