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一氧化氮自由基(nitric oxide radical,NO)具有强烈的母体及胎儿胎盘血液动力学调节作用。合体滋养细胞(SC)由细胞滋养细胞(CC)合体化而形成。作者采用免疫组化法检测了早孕期绒毛内滋养细胞及足月妊娠羊膜胎盘基底板之绒毛外滋养细胞的内皮一氧化氮合成酶(e NOS)的表达,并作滋养细胞体外培养,观察CC合体化过程中的e NOS表达。 方法:收集孕8周自然宫内胚胎死亡清宫物的绒毛组织及足月妊娠分娩后的绒毛,羊膜、绒毛膜、蜕膜组织,于液氮-70℃保存备用。按Bax法提取足
Nitric oxide radical (NO) has a strong maternal and fetal placental hemodynamic regulation. Syncytiotrophoblasts (SC) are formed by the syncytialization of cytotrophoblasts (CCs). The author used immunohistochemistry to detect the expression of endothelial nitric oxide synthase (eNOS) in trophoblasts in early villus chorionic villi and full-term pregnancy amniotic placenta basal plate and trophoblast cultured in vitro. CC ENOS expression during syncytia. Methods: The chorionic tissues and the chorion, amniotic membrane, chorion and decidua after the pregnancy were collected and stored in liquid nitrogen at 70 ℃ for 8 weeks. According to Bax extraction foot