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目的:观察δ阿片受体激动剂DADLE对全脑缺血再灌注大鼠大脑组织结构病理变化的影响,探讨DADLE在脑复苏中的神经保护作用。方法:50只SD大鼠随机分为5组,每组各10只:①假手术组(N):不制模不干预;②模型组(I/R):单纯制作全脑缺血再灌注模型;③DADLE预处理组(D1):在缺血前给予DADLE;④DADLE缺血后处理组(D2):在缺血后给予DADLE;⑤DADLE再灌注期处理组(D3):在再灌注早期给予DADLE。实验结束后,取大脑组织进行形态学检查。结果:全脑缺血再灌注大鼠大脑出现大量神经元坏死、胞浆浓缩、核固缩现象。DADLE各处理组缺血再灌注损伤的病理改变明显减轻。模型组和DADLE各处理组海马神经元细胞密度均低于假手术组(P<0.01),而DADLE各处理组海马神经元细胞密度高于模型组(P<0.01),DADLE各处理组之间海马神经元细胞密度差异没有统计学意义(P>0.05)。结论:δ阿片受体激动剂DADLE对大鼠全脑缺血再灌注损伤具有一定的保护作用。
OBJECTIVE: To observe the effect of DADLE, a δ opioid receptor agonist, on the pathological changes of brain tissue after global cerebral ischemia-reperfusion in rats and to explore the neuroprotective effect of DADLE in cerebral resuscitation. Methods: Fifty Sprague-Dawley rats were randomly divided into five groups with 10 rats in each group: ① Sham operation group (N): no intervention without modeling; ② model group (I / R): simple preparation of global cerebral ischemia-reperfusion DADLE was given before ischemia; DADLE was given to ischemic postconditioning group (D2): DADLE was given after ischemia; ⑤DADLE reperfusion group (D3): DADLE was given at the early stage of reperfusion . After the experiment, take the brain tissue for morphological examination. Results: A large number of neuron necrosis, cytoplasm condensation and nuclear pyknosis occurred in the brain of rats with global brain ischemia-reperfusion. The pathological changes of ischemia-reperfusion injury in DADLE treatment groups were significantly reduced. The density of hippocampal neurons in model group and DADLE treatment group was lower than that in sham operation group (P <0.01), while the density of hippocampal neurons in DADLE treatment group was higher than that in model group (P <0.01) There was no significant difference in the density of hippocampal neurons (P> 0.05). Conclusion: δ opioid receptor agonist DADLE has a protective effect on global cerebral ischemia-reperfusion injury in rats.