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Aim:To evaluate the effects of global system for mobile communications (GSM)1800 MHz microwaves on dendritic filopodia,dendritic arborization,and spinematuration during development in cultured hippocampal neurons in rats.Methods:The cultured hippocampal neurons were exposed to GSM 1800 MHz microwaveswith 2.4 and 0.8 W/kg,respectively,for 15 min each day from 6 days in vitro (DIV6)to DIV14.The subtle structures of dendrites were displayed by transfection withfarnesylated enhanced green fluorescent protein (F-GFP) and GFP-actin on DIV5into the hippocampal neurons.Results:There was a significant decrease in thedensity and mobility of dendritic filopodia at DIV8 and in the density of maturespines at DIV14 in the neurons exposed to GSM 1800 MHz microwaves with2.4 W/kg.In addition,the average length of dendrites per neuron at DIV10 andDIV14 was decreased,while the dendritic arborization was unaltered in theseneurons.However,there were no significant changes found in the neurons ex-posed to the GSM 1800 MHz microwaves with 0.8 W/kg.Conclusion:Thesedata indicate that the chronic exposure to 2.4 W/kg GSM 1800 MHz micro-waves during the early developmental stage may affect dendritic developmentand the formation of excitatory synapses of hippocampal neurons in culture.
Aim: To evaluate the effects of global system for mobile communications (GSM) 1800 MHz microwaves on dendritic filopodia, dendritic arborization, and spinematuration during development in cultured hippocampal neurons in rats. Methods: The cultured hippocampal neurons were exposed to GSM 1800 MHz microwaves with 2.4 for DIV14. The subtle structures of dendrites were displayed by transfection with enhanced green fluorescent protein (F-GFP) and GFP-actin on DIV5into the hippocampal neurons. Results: There was a significant decrease in the intensity and mobility of dendritic filopodia at DIV8 and in the density of matures pines at DIV14 in the neurons exposed to GSM 1800 MHz microwaves with 2.4 W / kg. In addition, the average length of dendrites per neuron at DIV10 and DIV14 was decreased, while the dendritic arborization was unaltered in these neurons. Yet, there were no significant changes found in the neurons ex-posed to t he GSM 1800 MHz microwaves with 0.8 W / kg. Conclusion: Thesedata indicate that the chronic exposure to 2.4 W / kg GSM 1800 MHz micro-waves during the early developmental stage may affect dendritic development and the formation of excitatory synapses of hippocampal neurons in culture.