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以玉米自交系L26和095组配的F2世代为作图群体,采用SSR分子标记技术和复合区间作图法对玉米茎粗等7个株型性状进行基因定位。共检出21个QTL,其中茎粗检测到1个位点(qSD1),穗位高、株高均检测到3个QTL位点(qEH1-qEH3、qPH1-qPH3),雄穗分枝数检测到5个QTL位点(qTBN1-qTBN5),叶片数检测到4个QTL位点(qLN1-qLN4),叶型系数检测到3个QTL位点(qLSC1-qLSC3),叶向值检测到2个QTL位点(qLOV1-qLOV2)。21个QTL中,qTBN1、qTBN4、qLN1、qLN3、qLN4这5个QTL解释表型变异率超过30%,表现出明显的主效QTL效应。研究还发现,有5个影响不同性状的QTL位于染色体上相同标记区间内或与相同标记连锁,分为Ch3-2和Ch8-1两个区段,表现出了成簇分布的特性。
F2 generations of maize inbred lines L26 and 095 were used as mapping population. SSR markers and composite interval mapping were used to map the 7 plant type traits in maize. A total of 21 QTLs were detected, of which 1 locus was detected by stem diameter (qSD1), 3 QTL loci (qEH1-qEH3, qPH1-qPH3) Four QTL loci (qLN1-qLN4) were detected in five QTL loci (qTBN1-qTBN5), three QTL loci (qLSC1-qLSC3) were detected in leaf type and two QTL locus (qLOV1-qLOV2). Among the 21 QTLs, five QTLs qTBN1, qTBN4, qLN1, qLN3 and qLN4 explained that the phenotypic variation rate exceeded 30% and showed a significant QTL effect. The study also found that five QTLs affecting different traits were located in the same marker interval on the chromosome or linked with the same marker, which was divided into two segments, Ch3-2 and Ch8-1, showing clustered distribution.