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目的探讨番茄红素对饮水性氟染毒小鼠氧化应激损伤与神经行为影响及可能机制。方法成年雄性C57BL/6小鼠60只,随机分为对照组、染氟组、番茄红素组、番茄红素低、中、高剂量干预组,每组10只;连续处理6个月后,氟离子选择电极法测定尿氟、血氟和脑氟含量;化学比色法检测血液和海马组织中丙二醛(MDA)含量及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)活力;水迷路实验分析小鼠神经行为学改变。结果与对照组比较,染氟组小鼠体重下降,尿氟、血氟和脑氟含量[分别为(3.76±1.60)、(19.26±0.62)、(1.19±0.12)mg/kg]升高(P<0.05),血液和海马组织中MDA含量显著增加、SOD、GSH-Px和CAT酶活力下降(P<0.05);与染氟组比较,番茄红素高剂量干预组尿氟、血氟和脑氟含量[分别为(2.06±0.47)、(13.40±0.35)、(0.52±0.21)mg/kg]明显下降(P<0.05),血液和海马组织中MDA含量降低、SOD、GSH-Px和CAT酶活力升高(P<0.05);与对照组比较,染氟组小鼠平均逃逸时间和靶象限活动时间[分别为(20.84±0.72)、(16.11±0.77)s]延长(P<0.05);与染氟组比较,番茄红素高剂量干预组小鼠平均逃逸时间和靶象限活动时间[分别为(14.01±0.82)、(7.38±0.37)s]下降(P<0.05)。结论番茄红素能有效降低饮水性氟染毒小鼠氧化应激损伤及提高小鼠学习记忆能力,对饮水性氟中毒具有一定保护作用。
Objective To investigate the effects of lycopene on oxidative stress and neurobehavioral changes in drinking water-exposed mice and its possible mechanism. Methods Sixty adult male C57BL / 6 mice were randomly divided into control group, fluoride group, lycopene group, low, medium and high dose of lycopene intervention group, with 10 rats in each group. After continuous treatment for 6 months, Fluoride, Fluoride and Fluoride were measured by fluoride ion selective electrode method. The levels of malondialdehyde (MDA) and superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activity. The mice’s neurobehavioral changes were analyzed by water labyrinth test. Results Compared with the control group, the body weight of the mice in the fluoride-exposed group was decreased, and the contents of urinary fluoride, blood fluoride and brain fluoride were significantly increased (3.76 ± 1.60, 19.26 ± 0.62, 1.19 ± 0.12 mg / kg, P <0.05). The contents of MDA, SOD, GSH-Px and CAT in blood and hippocampus were significantly decreased (P <0.05) The content of MDA in blood and hippocampus decreased, while the content of SOD, GSH-Px, and the content of MDA in blood and hippocampus were significantly decreased (P <0.05) (P <0.05). Compared with the control group, the mean escape time and target quadrant activity time of the mice in the fluorinated group [(20.84 ± 0.72) and (16.11 ± 0.77) s] ). Compared with the fluoride-exposed group, the mean escape time and the target quadrant time [(14.01 ± 0.82) and (7.38 ± 0.37) s] in high-dose lycopene intervention group decreased (P <0.05). Conclusions Lycopene can effectively reduce the oxidative stress injury and improve the learning and memory abilities of mice exposed to fluoride-poisoned water, and has some protective effects on drinking water fluorosis.