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以斑点杂交法测定染石英尘后7、14、21、30及60天时大鼠肺组织中前胶原mRNA水平,与正常肺组织相比,显示在染尘后7天时,proα_1(Ⅱ)mRNA显著增加,14至21天时达高峰。30天后处于平稳增高水平。proα_1(Ⅰ)与proα_2(Ⅰ)mRNA在染尘后14天才开始显著增高,30天时达最高值。表明在砂肺病变中,Ⅰ、Ⅱ型胶原的基因表达都有增加,但在时相上有不同。一种自染石英尘大鼠肺泡巨噬细胞中提取的致纤维化因子可特异地刺激2BS细胞中Ⅰ、Ⅱ型胶原基因的表达,对矽肺病变中胶原基因的表达有重要的调节作用。放线菌素D可抑制胶原基因的转录。
The level of procollagen mRNA in rat lung tissue at 7, 14, 21, 30 and 60 days after spotted quartz dust was determined by dot blot hybridization. Compared with normal lung tissue, proα_1 (Ⅱ) mRNA was significantly increased at 7 days after dusting Increase, reaching a peak between 14 and 21 days. After 30 days in a steady increase. The mRNA levels of proα_1 (Ⅰ) and proα_2 (Ⅰ) began to increase significantly at 14 days after exposure to dust, reaching the highest level at 30 days. The results showed that in sand-lung disease, the gene expression of type I and type II collagen increased, but it was different in time. A fibrotic factor extracted from alveolar macrophages of infected quartz dust rats can specifically stimulate the expression of type I and type II collagen genes in 2BS cells and play an important regulatory role in the expression of collagen genes in silicotic lesions. Actinomycin D inhibits collagen gene transcription.