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目的：将弓形虫ＺＳ１株主要表膜抗原（Ｐ３０）基因插入到昆虫杆状病毒基因组中，为在昆虫细胞或昆虫体内表达及生产Ｐ３０作准备。方法：先将Ｐ３０基因亚克隆到转移载体质粒ｐＳＸ－ＩＶＶＩ＋Ｘ３中，然后用此重组载体质粒ＤＮＡ与亲本株病毒ＤＮＡ共转染培养的草地夜蛾细胞，经空斑纯化筛选出重组病毒株。结果：已得到含Ｐ３０基因的重组杆状病毒ＴｎＮＰＶ－Ｐ３０。结论：本文已将弓形虫ＺＳ１株Ｐ３０基因亚克隆到昆虫杆状病毒ＴｎＮＰＶ中。
OBJECTIVE: To insert the major surface antigen (P30) gene of Toxoplasma gondii ZS1 into the genome of insect baculoviruses in preparation for the expression and production of P30 in insect cells or insects. Methods: The P30 gene was subcloned into the transfer vector pSX-IVVI + X3. Then, the recombinant plasmid was co-transfected with the DNA of the parental strain and the recombinant plasmid was selected by plaque purification. Results: Recombinant baculovirus TnNPV-P30 containing P30 gene was obtained. Conclusion: The P30 gene of Toxoplasma gondii ZS1 was subcloned into the insect baculovirus TnNPV.