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垂穗披碱草(Elymus nutans)和老芒麦(Elymus sibiricus)皆为花序下垂类披碱草属物种,高海拔地区的E.sibiricus的部分变异类型也具有小穗紧密排列等特征,与E.nutans在形态学性状上具有较多的交叉,造成野外种质资源采集时进行直接的田间鉴定存在困难。本研究利用12对小麦族SSR引物对8份垂穗披碱草和10份老芒麦种质进行遗传变异和物种鉴定分析,UPGMA聚类分析表明供试材料明显可依据物种差异划分成两大类,主向量分析(PCo A)与聚类分析的结果保持一致。种质间遗传相似系数分析和分子方差分析(AMOVA)也表明种间变异远高于种内。另外,本研究筛选出3对引物,ESGS79和ESGS155能够在垂穗披碱草材料中扩增出特异性条带,Xgwm311能够在老芒麦材料中扩增出特异性条带,这3对引物能够作为区分垂穗披碱草和老芒麦的依据,为野外种质资源的收集以及田间育种工作提供指导。
Elymus nutans and Elymus sibiricus are all inflorescences dwarfing Elymus species. Partial variation types of E.sibiricus at high altitude also have such features as closely-spaced spikelets, There was more crosses between morphology and morphological traits of nutnut, which made it difficult to identify field germplasm directly in the field. In this study, 12 pairs of SSR primers were used to analyze the genetic variation and species identification of 8 Elymus nigrum and 10 Eumhirts indica germplasms. UPGMA cluster analysis indicated that the tested materials were obviously divided into two major species The class, principal component analysis (PCo A) is consistent with the results of cluster analysis. Genetic similarity coefficient analysis and molecular variance analysis (AMOVA) also showed that the interspecific variation was much higher than that within the species. In addition, three pairs of primers were screened in this study, ESGS79 and ESGS155 could amplify specific bands in Elymus nutans, and Xgwm311 could amplify specific bands in Elymus sibiricus. The three pairs of primers Can be used as a basis for distinguishing Elymus nigrum and Erigeron barley as guidance for the collection of field germplasm resources and field breeding.