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目的 :研究 2 -乙基 - 3-羟基 - 6-苯硫基 - 4 ( 1H) -吡啶酮 (HPP)对四氯化碳 (CCl4 )、硫代乙酰胺 (TAA)及D -氨基半乳糖 (D -GalN)引发的小鼠急性肝损伤的影响 .方法 :用CCl4 、TAA、D -GalN引发动物急性肝损伤 ,HPP通过口服给药 ,测定动物血清中谷丙转氨酶 (GPT)、谷草转氨酶 (GOT)、谷胱甘肽过氧化物酶 (GSH -Px)、超氧化物歧化酶 (SOD)的活性 ,用硫代巴比妥酸法测定肝组织中丙二醛 (MDA)含量 .结果 :HPP 1.6mg·kg- 1、6.4mg·kg- 1显著抑制动物血清中GPT、GOT活性的升高 ,显著抑制GSH -Px ,SOD活性的降低且能降低肝组织中脂质过氧化产物MDA含量的升高 ,同时HPP对CCl4 所致大鼠急性肝损伤也有一定的抑制作用 ,实验表明HPP对正常小鼠血清及肝脏GPT活力无明显影响 .结论 :HPP对动物急性肝损伤有明显的抑制作用
Aims: To investigate the effect of 2 - ethyl - 3 - hydroxy - 6 - phenylthio - 4 (1H) - pyridone (HPP) on carbon tetrachloride (CCl4), thioacetamide (TAA) and D - galactosamine (D-GalN) -induced acute liver injury in mice.Methods: Acute hepatic injury was induced by CCl4, TAA and D-GalN in rats, and oral administration of HPP was performed to determine the levels of GPT and AST (aspartate aminotransferase GOT), glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) were determined by enzyme-linked immunosorbent assay (ELISA) HPP 1.6mg · kg-1 and 1.4mg · kg-1 significantly inhibited the increase of GPT and GOT activity in serum, and significantly inhibited the activity of GSH-Px and SOD and the content of lipid peroxidation products in liver tissue , While HPP also inhibited CCl4-induced acute liver injury in some extent.Experiments showed that HPP had no significant effect on GPT activity in serum and liver of normal mice.Conclusion: HPP has a significant inhibitory effect on acute liver injury in animals