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目的:建立一个经济有效的方法用于淡竹叶Lophatherum gracile中槲皮素-3-O-葡萄糖苷的分离纯化。方法:采用高速逆流色谱(high-speed counter-current chromatography,HSCCC)进行分离纯化,所用溶剂体系为乙酸乙酯-正丁醇-水(2∶1∶3),其上相和下相是根据UNIFAC数学模型单独配制,并与传统配制方法配制所得溶剂系统的分离效率进行比较;分离所得产物的纯度经HPLC检测,结构经MS,UV,NMR鉴定。结果:HSCCC使用UNIFAC数学模型单独配制的溶剂系统成功的从竹叶中制备出纯度高于99%的槲皮素-3-O-葡萄糖苷,与传统溶剂系统配制方法相比,节省了乙酸乙酯、正丁醇和水的用量。结论:在淡竹叶中槲皮素-3-O-葡萄糖苷的HSCCC分离过程中,使用UNIFAC单独配制的溶剂系统既可保证较好分离效果,又可节省大量的有机溶剂,减少了资源浪费,益于环保。
OBJECTIVE: To establish a cost-effective method for the isolation and purification of quercetin-3-O-glucoside from Lophatherum gracile. Methods: The high-speed counter-current chromatography (HSCCC) was used for the isolation and purification. The solvent system used was ethyl acetate-n-butanol-water The UNIFAC mathematical model was prepared separately and compared with the separation efficiency of the solvent system prepared by the traditional preparation method. The purity of the isolated product was determined by HPLC and the structure was identified by MS, UV and NMR. Results: The solvent system prepared by HSCCC by UNIFAC mathematical model successfully prepared quercetin-3-O-glucoside with purity higher than 99% from bamboo leaves. Compared with the traditional solvent system preparation method, Esters, n-butanol and water. Conclusion: In the process of HSCCC separation of quercetin-3-O-glucoside in light bamboo leaves, the solvent system prepared by UNIFAC alone can not only ensure good separation effect but also save a large amount of organic solvent, reduce the waste of resources, Benefit from environmental protection.