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利用SSR技术分析了山西省审定玉米品种亲本自交系的遗传多样性及杂优类群。40对引物在83个自交系中共检测出232个等位基因,每个SSR位点的等位基因数为3~12个,平均为5.8个;平均多态信息量0.69,变幅为0.43~0.88。共检测出67个稀有等位基因和21个特有等位基因。SSR聚类分析可将自交系划分为6个类群,确定山西省玉米审定品种的杂优模式有15种。研究表明,山西省玉米审定品种双亲自交系的遗传多样性丰富,很多自交系具有独特基因,它们可能具有一定的特异性。对自交系杂优类群的划分解决了当前育种中出现的自交系血缘不清问题,对山西省主要玉米品种杂优利用模式的分析可为山西省乃至全国的玉米育种提供科学依据。
The genetic diversity and heterosis groups of inbred lines of maize validated in Shanxi Province were analyzed by using SSR technique. A total of 232 alleles were detected in 83 inbred lines with 40 pairs of primers. The number of alleles per SSR locus ranged from 3 to 12 with an average of 5.8. The average number of polymorphic information was 0.69 and the amplitude was 0.43 ~ 0.88. A total of 67 rare alleles and 21 specific alleles were detected. SSR cluster analysis can be divided into six groups of inbred lines, Shanxi Province to determine the validation varieties of corn hybrids have 15 species. Studies have shown that parents of inbred lines of maize validation varieties in Shanxi Province have rich genetic diversity, and many inbred lines have unique genes that may have certain specificity. The division of the inbreds inbred lines solved the problem of unclear kin in the current inbred lines. The analysis of the heterosis utilization pattern of the main maize varieties in Shanxi Province may provide a scientific basis for maize breeding in Shanxi Province and the whole country.