目的观察枸杞多糖(LBP)对加压诱导凋亡的视网膜神经节细胞(RGCs)钾K~+电流的影响,以探讨枸杞多糖对RGCs的保护作用。方法取SD乳鼠视网膜神经节细胞原代培养,分为对照组,加压组,LBP各剂量组(100,250,500,1 000μg·mL~(-1)),对照组常规培养6 d;加压组常规培养6 d后用自行设计的加压装置加压80 mm Hg,1 h;LBP各组常规培养5 d后,加入不同浓度的LBP共培养24 h后,再加压80 mm Hg,1 h。通过连续波长多功能微孔板检测仪检测各组RGCs线粒体的平均荧光强度值;通过全细胞膜片钳技术观察各组细胞膜电容(membrane capacitance,C_m)和钾(K~+)电流的变化。结果加压组C_m和RGCs线粒体荧光强度低于对照组,差异有统计学意义(P<0.05),LBP 250,LBP 500,LBP 1 000组RGCs线粒体荧光强度和C_m高于加压组(P<0.05,P<0.01)。电流记录显示,与对照组比较加压组外向K~+电流增加,用不同浓度的LBP预处理后,K~+电流增加都有抑制,且呈剂量依赖性(P<0.05,P<0.01)。结论枸杞多糖可抑制加压诱导的视网膜神经节细胞凋亡,其作用可能与抑制K~+电流有关。 Objective To observe the effects of LBP on potassium K + currents in retinal ganglion cells (RGCs) under pressure to investigate the protective effects of LBP on RGCs. Methods Primary cultured retinal ganglion cells of SD neonatal rats were divided into control group, pressure group and each LBP dose group (100, 250, 500 and 1000 μg · mL -1) After routine culture for 6 days, self-designed pressurization device was used to pressurize 80 mm Hg for 1 h. LBP groups were cultured for 5 days and then LBP of different concentrations were co-cultured for 24 h followed by 80 mm Hg and 1 h . The average fluorescence intensity of mitochondria in each group of RGCs was detected by continuous wave multi-function microplate detector. The changes of membrane capacitance (C_m) and potassium (K ~ +) currents were observed by whole-cell patch clamp technique. Results The mitochondrial fluorescence intensity of C_m and RGCs in pressure group was lower than that in control group (P <0.05). The fluorescence intensity and C_m of RGCs in LBP 250, LBP 500 and LBP 1000 group were higher than those in pressure group (P < 0.05, P <0.01). Current recordings showed that extrinsic K ~ + currents increased in the pressured group compared with the control group, and inhibited by KP + pretreatment with different concentrations of LBP (P <0.05, P <0.01) . Conclusion Lycium barbarum polysaccharide can inhibit the apoptosis of retinal ganglion cells induced by pressure, which may be related to the inhibition of K ~ + current.