目的观察亚低温联合依达拉奉对大鼠脑缺血再灌注损伤的保护作用。方法制备SD大鼠MCAO模型,梗死2h后再灌注,并分为假手术组、模型组、依达拉奉组及亚低温联合依达拉奉组(联合组),再灌注24 h后进行神经功能缺损评分、检测脑组织超氧化物歧化酶(SOD)、丙二醛(MDA)浓度、病理变化及大脑缺血面积并比较。结果 (1)依达拉奉组与联合组的神经功能缺损评分明显低于模型组,且联合组的神经功能缺损评分明显低于依达拉奉组(P均<0.01)。(2)与假手术组相比,模型组SOD活性降低、MDA含量增加(P<0.01);与模型组相比,依达拉奉组与联合组S0D活性增高、MDA含量降低(P<0.01),且联合组与依达拉奉组相比有显著性差异(P<0.01)。(3)模型组、依达拉奉组及联合组病理学检查均较假手术组差,但联合组好于依达拉奉组好于模型组(P<0.01)。(4)模型组、依达拉奉组及联合组脑缺血面积较假手术组增大,而联合组<依达拉奉组<模型组,差异均有统计学意义(P<0.01)。结论亚低温联合依达拉奉对脑缺血再灌注损伤有较好保护作用。 Objective To observe the protective effect of mild hypothermia combined with edaravone on cerebral ischemia-reperfusion injury in rats. Methods SD rats MCAO model was established and reperfused 2 hours after infarction. The rats were divided into sham operation group, model group, edaravone group and mild hypothermia combined with edaravone group (combination group) Function deficit score, the content of superoxide dismutase (SOD), malondialdehyde (MDA), pathological changes and the area of ​​cerebral ischemia were detected and compared. Results (1) The score of neurological deficit in edaravone group and combined group was significantly lower than that in model group, and the score of neurological deficit in combined group was significantly lower than that of edaravone group (all P <0.01). (2) Compared with the sham operation group, SOD activity and MDA content in the model group decreased (P <0.01). Compared with the model group, the SOD activity and the content of MDA in the edaravone group and the combination group decreased ), And there was a significant difference between the combined group and the edaravone group (P <0.01). (3) The pathological changes in model group, edaravone group and combined group were worse than those in sham operation group, but the combination group was better than edaravone group (P <0.01). (4) The area of ​​cerebral ischemia in model group, edaravone group and combined group increased more than that in sham operation group, but there was significant difference in combined group