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为探讨马铃薯不同器官中抗坏血酸(AsA)含量及其代谢相关酶活性关系,研究了马铃薯幼叶、功能叶、老叶、茎和块茎中AsA和其氧化态脱氢抗坏血酸(DHA)的含量与L-半乳糖-1,4-内酯脱氢酶(GalLDH)、脱氢抗坏血酸还原酶(DHAR)、谷胱甘肽还原酶(GR)、抗坏血酸过氧化物酶(APX)、抗坏血酸氧化酶(AO)和单脱氢抗坏血酸还原酶(MDHAR)等6种酶活性之间的相关性。结果表明,马铃薯AsA在幼叶和块茎中含量很高。叶片和茎的抗坏血酸库(AsA与DHA之和)水平与GalLDH活性显著相关,而AsA含量与DHAR活性显著相关,DHA含量与APX活性显著相关。说明在马铃薯幼叶中高含量的AsA可能由于GalLDH和DHAR的高活性;而块茎中AsA的积累,主要来自于叶片的运输和DHAR催化的DHA再生。
In order to explore the relationship between the content of AsA and the activity of enzymes related to its metabolism in different organs of potato, the content of AsA and its oxidative form dehydroascorbic acid (DHA) in young leaves, functional leaves, old leaves, stems and tubers of potato were studied. Galactose-1,4-lactone dehydrogenase (GalLDH), dehydroascorbate reductase (DHAR), glutathione reductase (GR), ascorbate peroxidase (APX), ascorbate oxidase ) And monodehydroascorbate reductase (MDHAR) and other six kinds of enzyme activity. The results showed that AsA in potato was high in young leaves and tubers. Ascorbate pools (sum of AsA and DHA) of leaves and stems were significantly correlated with GalLDH activity, while AsA content was significantly correlated with DHAR activity. DHA content was significantly correlated with APX activity. Indicating that high levels of AsA in young leaves of potato may be due to the high activity of GalLDH and DHAR, whereas accumulation of AsA in tubers is mainly due to leaf transport and DHAR-catalyzed DHA regeneration.