论文部分内容阅读
目的探讨丹参酮ⅡA(tanshinoneⅡA,TanⅡA)抗人胃癌MGC-803作用及其可能作用机制。方法通过细胞形态学观察、生长曲线绘制和克隆实验观察TanⅡA对MGC-803细胞增殖的影响;应用Hoechest33258和PI双染法观察TanⅡA对MGC-803细胞凋亡的影响;采用荧光分光光度计检测TanⅡA对MGC-803细胞细胞内钙([Ca2+]i)及线粒体膜电位的影响;RT-PCR检测TanⅡA对MGC-803细胞Bad和金属硫蛋白-1A(MT)-1A mRNA表达的影响。结果 TanⅡA能抑制MGC-803细胞增殖,且随TanⅡA剂量的增加和作用时间的延长而增强。TanⅡA作用MGC-803细胞24 h后,MGC-803细胞出现染色质聚集等典型的凋亡形态学改变,且随TanⅡA剂量的增加,MGC-803细胞凋亡百分率逐渐增大。TanⅡA作用后,MGC-803细胞的细胞内钙升高、线粒体膜电位降低、Bad mRNA表达增加、MT-1A mRNA表达下调。结论 TanⅡA具有诱导MGC-803细胞凋亡作用,可能与钙依赖性通路和MT-1A表达下调有关。
Objective To investigate the anti-human gastric cancer MGC-803 effect of tanshinoneⅡA (TanⅡA) and its possible mechanism. Methods The effect of Tan II A on the proliferation of MGC-803 cells was observed by cell morphology, growth curve and cloning experiments. The effect of Tan II A on the apoptosis of MGC-803 cells was observed by Hoechest 33258 and PI double staining. The fluorescence intensity of Tan II A was detected by fluorescence spectrophotometer (Ca2 +) i and mitochondrial membrane potential in MGC-803 cells. The effect of TanⅡA on the expression of Bad and metallothionein-1A (MT) -1A mRNA in MGC-803 cells was detected by RT-PCR. Results TanⅡA inhibited the proliferation of MGC-803 cells and increased with the increase of TanⅡA dose and the prolongation of action time. The apoptosis of MGC-803 cells induced by TanⅡA was observed 24 h after MGT-803 cells were treated with TanⅡA. The apoptosis percentage of MGC-803 cells increased with the increase of TanⅡA dose. After Tan II A treatment, MGC-803 cells increased intracellular calcium, mitochondrial membrane potential decreased, Bad mRNA expression increased, and MT-1A mRNA expression decreased. Conclusion TanⅡA can induce the apoptosis of MGC-803 cells, which may be related to the down-regulation of calcium-dependent pathway and MT-1A expression.