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目的:进行复方阿胶补血合剂质量标准的研究。方法:用薄层色谱法对复方阿胶补血合剂中的何首鸟、黄精、狗脊、陈皮、进行定性鉴别。利用HPLC法对制剂中的大黄素进行了含量测定,流动相:甲醇-0.5%HCIO4(80:20);流速:0.5L/min;柱温:40℃;检测波长:254 nm。结果:复方阿胶补血合剂中大黄素的平均含量不低于(6.97±0.08)μg/mL,其平均回收率为96,96%,RSD为1.72%(N=5)。结论:该方法可为复方阿胶补血合剂的质量评价提供科学依据。
Objective: To study the quality standard of compound Ejiao Buxue mixture. METHODS: TLC was used to qualitatively identify Ho Shou Zhi, Huang Jing, Zhi Ji, and Chen Pi in Compound Ejiao Buxue Mixture. The content of emodin in the preparation was determined by HPLC. The mobile phase was methanol-0.5% HCIO4 (80:20); flow rate: 0.5L/min; column temperature: 40°C; detection wavelength: 254 nm. Results: The average content of emodin in compound Ejiao Buxue Mixture was not lower than (6.97±0.08)μg/mL. The average recovery rate was 96,96% and the RSD was 1.72% (N=5). Conclusion: This method can provide scientific basis for the quality evaluation of compound Ejiao Buxue mixture.