建立了高效液相色谱法同时测定卫肺物片中利福平、异烟肼、吡嗪酰胺的含量。采用Ｃ１８柱，以００１ｍｏｌ／Ｌ磷酸二氢铵甲醇为流动相，检测波长２５４ｎｍ，柱温３５℃，流速１０ｍｌ／ｍｉｎ。利福平、异烟肼、吡嗪酰胺的线性范围分别为：２７～４３２μｇ／ｍｌ（ｒ＝０９９９５，ｎ＝５），１５６２５～２５０μｇ／ｍｌ（ｒ＝０９９９８，ｎ＝５），６２５～１０００μｇ／ｍｌ（ｒ＝０９９９３，ｎ＝５）。加样回收理想，方法准确、简便、快速。 A HPLC method was established for the simultaneous determination of rifampicin, isoniazid and pyrazinamide in Weifei tablets. A C18 column was used. The mobile phase was 001mol / L ammonium dihydrogen phosphatemethanol. The detection wavelength was 254nm, the column temperature was 35 ℃, and the flow rate was 1.0  0ml / min. The linear ranges of rifampin, isoniazid and pyrazinamide were 27 ~ 432μg / ml (r = 09995, n = 5), 15625 ~ 250μg / ml (r = 09998, n = 5), 625 ~ 1000μg / ml (r = 09993, n = 5). Ideal for sample recovery, the method is accurate, simple and fast.