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精子发生是一个多基因参与的复杂的生理过程,虽然人们克隆了一些与精子发生相关的基因,但迄今为止,人们对精子发生的分子机制了解有限,因而克隆相关的新基因,并研究其在理论上和实践上的功能仍然十分重要。该文从人类睾丸cDNA文库出发,以小鼠精子发生相关基因mTSARG7基因为电子探针,得到1个与人类的精子发生相关的新基因TSARG7(GenBank登录号为AY513610)。该基因的cDNA全长为2463bp,含有12个外显子和11个内含子,定位在人类8号染色体8p11.21上。TSARG7编码的蛋白质含有456个氨基酸,分子量为56.295kDa,等电点为9.13,为胞浆中非分泌性蛋白,具有磷酸酰基转移酶(PISC)的结构域,属于酰基转移酶家族的新成员,该家族成员具有脂质合成的功能。TSARG7和mTSARG7,TSARG7和Au041707分别具有97%的同源性。该基因在睾丸中特异表达,亚细胞定位在胞浆中表达。TSARG7mRNA在13岁的睾丸中开始表达,并且随着精子的发生和性成熟而稳定增加,热应急实验显示该基因的表达与温度相关。综上所述,该文克隆了人的1个新基因,该基因与精子的发生和性成熟相关。
Although spermatogenesis is a complex physiological process involved in multiple genes, although some genes related to spermatogenesis have been cloned, so far, there is limited understanding of the molecular mechanism of spermatogenesis, so new genes related to spermatogenesis are cloned and studied The theoretical and practical functions are still very important. Based on the human testis cDNA library, the mTSARG7 gene of mouse spermatogenesis was used as an electron probe to obtain a new gene TSARG7 (GenBank accession number: AY513610) related to human spermatogenesis. The full-length cDNA of this gene is 2463 bp, contains 12 exons and 11 introns, and is located on human chromosome 8p11.21. TSARG7 protein contains 456 amino acids, molecular weight of 56.295kDa, isoelectric point of 9.13, is a non-secreted protein in the cytoplasm, with phosphotransferase (PISC) domain, belonging to acylase family of new members, Members of this family have lipid synthesis functions. TSARG7 and mTSARG7, TSARG7 and Au041707 have 97% homology, respectively. The gene is specifically expressed in the testis and subcellular localization is expressed in the cytoplasm. TSARG7 mRNA began to express in 13-year-old testes and steadily increased with spermatogenesis and sexual maturation. Heat-shock experiments showed that the expression of this gene correlated with temperature. In summary, the paper cloned a human new gene, the gene associated with the occurrence and sexual maturation of sperm.