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目的介绍一种诱导神经干细胞向神经元定向分化的方法。方法采用选择性无血清培养液培养大鼠神经干细胞。纯化培养2或3代后,将其接种于条件培养液中,诱导其向神经元定向分化。用倒置显微镜观察形态学变化,用NSE免疫细胞化学法检测其向神经元分化情况。结果条件培养液可有效诱导神经干细胞向神经元定向分化。将神经球消化成单细胞后接种,不但分化过程比神经球接种更同步,而且可提高其向神经元分化的比例。结论本方法可稳定高效地诱导神经干细胞向神经元定向分化。
Objective To introduce a method of inducing neural stem cells to differentiate into neurons. Methods Neural stem cells were cultured in selective serum - free medium. After two or three passages of purification, they were inoculated into conditioned medium to induce their directional differentiation into neurons. Morphological changes were observed with an inverted microscope, and neuronal differentiation was detected by NSE immunocytochemistry. Results Conditioned culture medium can effectively induce neural stem cells to differentiate into neurons. The neurospheres digested into single cells after inoculation, not only the process of differentiation is more synchronized than the neurospheres, and can improve the proportion of neurons differentiated. Conclusion This method can stably and efficiently induce neural stem cells to differentiate into neurons.