论文部分内容阅读
目的 :了解冠心病患者外周循环单核细胞组织因子 (TF)表达与冠心病病情的关系 ;观察溶栓治疗对急性心肌梗死患者单核细胞TF表达的影响。方法 :测定了 2 2例稳定型心绞痛 (SA)患者 ,2 1例不稳定型心绞痛(UA)患者 ,1 5例急性心肌梗死 (AMI)患者用尿激酶溶栓前及溶栓后 ,1 8例健康人外周循环单核细胞组织因子阳性率。单核细胞组织因子阳性率测定用免疫荧光染色及流式细胞术。结果 :SAP组 ,UAP组和AMI组单核细胞TF阳性率高于对照组 ,UAP组和AMI组单核细胞TF阳性率高于SAP组 ,单核细胞TF阳性率与冠心病病情显著相关。急性心肌梗死患者溶栓后 2h单核细胞TF阳性率升高。结论 :外周血单核细胞TF的表达与冠心病病情严重程度密切相关 ;急性心肌梗死患者溶栓治疗后单核细胞TF的表达进一步增强 ,这可能是溶栓后凝血酶活化的原因之一。
OBJECTIVE: To investigate the relationship between the expression of TF and the severity of coronary heart disease (CHD) in patients with coronary heart disease (CHD) and the effect of thrombolytic therapy on TF expression in monocytes in patients with acute myocardial infarction. Methods: Twenty-two patients with stable angina pectoris (SA) and 21 patients with unstable angina pectoris (UA) were enrolled in this study. Before urokinase thrombolytic therapy and in 15 patients with acute myocardial infarction (AMI), 18 Cases of healthy people peripheral blood mononuclear tissue factor positive rate. Monocyte tissue factor positive rate was determined by immunofluorescence staining and flow cytometry. Results: The positive rate of TF in SAP group, UAP group and AMI group was higher than that in control group. The positive rate of TF in UAP group and AMI group was higher than that in SAP group. The positive rate of TF in monocytes was significantly correlated with coronary heart disease. 2 h after thrombolysis in patients with acute myocardial infarction increased the positive rate of monocyte TF. CONCLUSION: The expression of TF in peripheral blood mononuclear cells is closely related to the severity of coronary heart disease. The expression of TF in mononuclear cells is further increased after thrombolytic therapy in patients with acute myocardial infarction, which may be one of the reasons for thrombin activation after thrombolysis.