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目的 观察微丝对豚鼠胃窦环行平滑肌细胞钾电流的影响 ,并且探讨肌动蛋白微丝是否参与低渗牵张增强豚鼠胃窦环行平滑肌细胞钾电流的过程。方法 本实验用Ⅱ型胶原酶急性分离单个豚鼠胃窦部环行平滑肌细胞 ,采用传统全细胞模式的膜片钳技术 ,以微丝的解聚剂细胞松弛素B(Cytocha
Objective To observe the effects of actin filaments on potassium currents in antral smooth muscle cells of guinea pig and to investigate whether actin microfilament is involved in hypotonic stretch-induced potassium current in antral smooth muscle cells of guinea-pig. Methods In this study, acute smooth muscle cells from the antrum of a single guinea pig were isolated from type Ⅱ collagenase by using the traditional whole-cell patch clamp technique. Cytochaeta