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一、试验材料板栗有原引于河北的遵化十一号、下庄二号,山东的金丰、郯城207、江苏的处暑红、广西的中果红油皮;日本栗有原产日本国的乙炊、银寄、岳王、金华、丹泽、筑波、石锤;本所自育的日本栗和板栗的人工杂交种银寄×遵化十一号以及代表丹东地区的杂种类型大栗树—红石一号。除本母树外,均系2—4年生以上幼树。二、试验方法1、过氧化物酶同功酶的制备:1982年8月、1983年5月、1984年3月,每批、每试样均取鲜栗芽一克,置于钵中,加蒸馏水
First, the test material Chestnut Originally introduced in Hebei Zunhua No. 11, Xiazhuang II, Shandong Jinfeng, Gaocheng 207, Jiangsu Shucu red, Guangxi red fruit skin; Japanese chestnut with Japanese origin B Yum, silver, Yue Wang, Jinhua, Danze, Tsukuba, stone hammer; the self-breeding of Japanese chestnut and chestnut hybrid Yin Yin Xunhua eleven and on behalf of Dandong hybrid type chestnut tree - red Stone one. In addition to the mother tree, are 2-4 years old saplings. Second, the test method 1, peroxidase isoenzyme preparation: 1982 August, 1983 May, 1984 March, each batch, each sample taken fresh chestnut one gram, placed in a bowl, Add distilled water