新生隐球菌主要感染细胞免疫缺陷病人,尤其是AIDS病患者。一般由呼吸道起肺部感染,甚至脑膜炎。因此肺和全身的防御功能对预防隐球菌感染是必要的。但其机制尚未阐明。 已知细胞免疫对宿主抗隐球菌感染起重要作用。CD_4~+T细胞对低毒力和高毒力隐球菌都有保护作用,而CD_8~+T细胞(以下简称CD_8)对低毒力有保护作用,但对致死性高毒力感染尤其是播散性感染致死性脑膜炎的作用还不清楚。本实验为此而设计。实验方法:体内注射抗-CD_8单克隆抗体并证实CD_8数和功能完全缺失(以定量及MLR法)的小鼠和注射IgG作对照的小鼠分别气管内和静脉内注射高毒力新生 Cryptococcus neoformans mainly infects patients with immunodeficiency, especially AIDS patients. The respiratory tract usually affects the lungs, even meningitis. Therefore, lung and systemic defenses are necessary to prevent cryptococcal infection. But its mechanism has not been clarified yet. Cellular immunity is known to play an important role in host anti-cryptococcal infection. CD_4 ~ + T cells have protective effects on both virulent and virulent Cryptococcus neoformans, while CD_8 ~ + T cells (hereinafter referred to as CD_8) have a protective effect on low virulence, but especially on virulent virulent infections The role of lethal meningitis in disseminated infections is not clear. This experiment is designed for this purpose. Experimental Methods: Mice injected with anti-CD_8 monoclonal antibody in vivo and confirmed that the number of CD8 and their functions were completely absent (both quantitative and MLR methods) and mice injected with IgG were injected intratracheally and intravenously with high virulence neonates