论文部分内容阅读
目的:探讨L-谷氨酰胺(L-Gln)对次声暴露下大鼠海马细胞凋亡率和坏死率的影响。方法:将60只雄性SD大鼠随机分为正常对照组、次声组、次声+药物组、药物→次声组,分别将次声组、次声+药物组、药物→次声组暴露于16Hz、130dB次声环境中,正常对照组也置于次声舱内,但期间不给予次声作用。经7d相应处理后测试各组大鼠海马细胞凋亡率和坏死率的变化。结果:与正常对照组大鼠相比,次声组海马细胞凋亡率和坏死率显著升高(P<0.01);与次声组相比,次声+药物组、药物→次声组,海马细胞凋亡率和坏死率显著降低(P<0.01);与药物→次声组相比,次声+药物组海马细胞凋亡率和坏死率降低,但差异无显著性意义(P>0.05)。结论:次声(16Hz/130dB,2h/d,共7d)作用可引起大鼠海马细胞凋亡及坏死,导致海马细胞损伤;L-Gln能够部分有效预防和保护次声对大鼠海马细胞损伤的作用。
Objective: To investigate the effect of L-Gln on the apoptosis rate and necrosis rate of rat hippocampus under infrasound. Methods: Sixty male Sprague-Dawley rats were randomly divided into normal control group, infrasound group, infrasound + drug group and drug → infrasound group. The infrasound group, infrasound + drug group and drug- infrasound group were exposed In the 16Hz, 130dB infrasound environment, the normal control group was also placed in the acoustic capsule, but during which no infrasound was given. After 7d corresponding treatment, the apoptosis rate and necrosis rate of hippocampus in each group were tested. Results: Compared with the control group, the rate of hippocampal apoptosis and necrosis in the infrasound group were significantly increased (P <0.01). Compared with the infrasound group, the infrasound + drug group, the drug- infrasound group, The apoptosis rate and necrosis rate of hippocampus in hippocampus were significantly decreased (P <0.01). Compared with those in infrasound + infrasound, hippocampal hippocampal apoptosis rate and necrosis rate were decreased, but there was no significant difference (P> 0.05 ). CONCLUSION: Infrasound (16Hz / 130dB, 2h / d for 7d) can induce apoptosis and necrosis of hippocampal cells in rat hippocampus, and lead to damage of hippocampal cells. L-Gln can partly prevent and protect infants from injury of hippocampal cells Role.