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目的:通过检测Netrin-I在正常足月妊娠、重度子痫前期、胎儿生长受限及重度子痫前期合并胎儿生长受限胎盘中的定位及表达,探讨Netrin-1与妊娠过程中胎盘形成及胎儿生长的关系。方法:分别采用免疫组织化学染色法、实时荧光定量逆转录聚合酶链反应(Real-time PCR)及免疫印迹法(Western blot)检测24例正常足月妊娠、18例重度子痫前期、15例胎儿生长受限及18例重度子痫前期合并胎儿生长受限胎盘中Netrin-1蛋白定位、含量及mRNA的表达水平。结果:Netrin-1定位于细胞滋养细胞及合体滋养细胞的胞浆。real-time PCR及免疫印迹法结果显示,与正常足月胎盘相比,重度子痫前期胎盘Netrin-1mRNA及蛋白水平稍有下降,但无统计学意义(P>0.05),但在胎儿生长受限组及重度子痫前期合并胎儿生长受限组中Netrin-1表达水平显著降低(P<0.01)。结论:孕期滋养细胞是Netrin-1的重要来源之一。Netrin-1参与了妊娠期胎盘形成的调控,可能在胎儿生长过程中发挥重要作用。
OBJECTIVE: To investigate the role of Netrin-1 in placenta formation and pregnancy during pregnancy, severe term preeclampsia, fetal growth restriction and severe preeclampsia with placental growth restriction The relationship between fetal growth. Methods: Twenty-four normal full-term pregnancies, 18 severe preeclampsia and 15 patients with severe preeclampsia were detected by immunohistochemical staining, Real-time PCR and Western blot respectively. Fetal growth restriction and 18 cases of severe preeclampsia placental growth restriction placenta Netrin-1 protein localization, content and mRNA expression levels. Results: Netrin-1 localized in the cytoplasm of cytotrophoblasts and syncytiotrophoblasts. Real-time PCR and Western blotting showed that there was a slight decrease in Netrin-1 mRNA and protein levels in severe preeclampsia compared with normal term placenta, but there was no significant difference (P> 0.05) The expression of Netrin-1 was significantly lower in the control group and severe preeclampsia combined with fetal growth restriction group (P <0.01). Conclusion: Trophoblast during pregnancy is one of the important sources of Netrin-1. Netrin-1 is involved in the regulation of placenta formation during pregnancy and may play an important role in fetal growth.