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目的:为掌握黄芪根腐病菌毒素滤液产生条件和探讨黄芪根腐病菌致病机制,以及为利用毒素进行种质抗原筛选奠定理论基础。方法:用培养皿滤纸发芽法和胚根生长抑制法研究了10株来源不同的黄芪根腐病菌茄病镰刀菌Fusarium solani毒素滤液的生物活性和专化性。结果:黄芪根腐病菌F.solani在不同培养条件下产生的毒素滤液对黄芪胚根均有较强的抑制作用。随着培养时间的延长,胚根抑制率逐渐增高,第12天时毒素滤液的抑制作用最强,抑制率在52.0%～92.0%。5～35℃下病菌所产毒素滤液均具有较强抑制作用,以25℃时毒素滤液的抑制率最高,其次为20,30℃。PSC培养液所产毒素滤液的抑制率较高,其次为PDB。光照条件对毒素滤液生物活性物质的影响不显著,但24h连续黑暗更有利于毒素滤液生物活性物质的产生。所有条件下产生的毒素滤液经灭菌后对黄芪胚根仍有很强的抑制作用,表现出较高的热稳定性。供试菌株HQM40培养12d后产生的毒素滤液对4种供试植物种子的胚根都具有抑制作用。结论:10株黄芪根腐病菌在不同培养条件下产生的毒素滤液生物活性强,对热稳定;产生毒素滤液的最佳条件是PSC培养液、25℃,24h黑暗条件下培养12d。HQM40菌株所产毒素滤液所含生物活性物质为非专化性毒素物质。
OBJECTIVE: To establish the conditions for the toxin filtrate of Astragalus root rot fungi and to explore the pathogenesis of root rot pathogen, and to lay a theoretical foundation for the screening of germ plasm antigens using toxins. Methods: The biological activity and specificity of Fusarium solani toxin filtrate from 10 different Astragalus root rot pathogens were studied by using the culture paper filter paper germination method and radicle growth inhibition method. Results: The toxin filtrate produced by Astragalus root rot fungi F. solani under different culture conditions had a strong inhibitory effect on Radix Astragali Radix. With the extension of culture time, the inhibition rate of radicle gradually increased. On the 12th day, the toxin filtrate had the strongest inhibitory effect with the inhibition rate of 52.0% -92.0%. The toxins filtrate produced by the bacteria at 5 ~ 35 ℃ had a strong inhibitory effect. The highest inhibitory rate of the toxin filtrate was at 25 ℃, followed by 20,30 ℃. The inhibition rate of toxin filtrate produced by PSC culture medium was higher, followed by PDB. The light conditions had no significant effect on the biologically active substances of the toxin filtrate, but 24h continuous darkness was more conducive to the production of biologically active substances in the toxin filtrate. The toxin filtrate produced under all conditions after sterilization has a strong inhibitory effect on the Radix Astragali Radix, showing high thermal stability. The toxin filtrate produced by the tested strain HQM40 for 12 days had an inhibitory effect on the radicle of the four tested plant seeds. Conclusion: 10 strains of Astragalus root rot bacteria produced in different culture conditions toxin filtrate strong biological activity, thermal stability; to produce the best toxin filtrate is PSC culture medium, 25 ℃, 24h dark conditions for 12 days. The biologically active substances contained in the toxin filtrate produced by the strain HQM40 are non-specific toxins.