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目的研究HIV-1基因突变株对艾滋病流行的关系,为控制艾滋病流行提供科学依据。方法分3个时段检测贵州省多地区192份HIV-1毒株样本,用套式PCR扩增其Env、Gag基因和测序分析,实验结果与贵州省艾滋病流行现状进行相关分析。结果贵州省HIV-I毒株经变异产生了CRF07-BC、CRF08-BC、CRF01-AE3种新的基因重组亚型毒株,亚型的产生、传播与贵州省艾滋病的暴发流行高度相关。结论 HIV-1毒株的突变或重组是加速贵州省艾滋病传播与流行的因素之一;HIV-1基因突变引起亚型改变先于HIV的暴发流行,及时监控HIV-1基因突变,可作为防控艾滋病暴发流行的先行指标之一。
Objective To study the relationship between HIV-1 mutant and AIDS epidemic in order to provide a scientific basis for controlling the HIV / AIDS epidemic. Methods A total of 192 samples of HIV-1 strains from various areas of Guizhou Province were detected in three periods. Envelopes and Gag genes were amplified by nested PCR and sequenced. The results were compared with the prevalence of HIV / AIDS in Guizhou Province. Results Mutations of HIV-I strains in Guizhou Province resulted in the production of three novel recombinant subtypes of CRF07-BC, CRF08-BC and CRF01-AE. The generation and transmission of subtypes were highly correlated with the outbreak of AIDS in Guizhou Province. Conclusion Mutation or recombination of HIV-1 strain is one of the factors that accelerate the transmission and epidemic of AIDS in Guizhou Province. HIV-1 gene mutation causes subtype change before HIV outbreak and timely monitoring of HIV-1 gene mutation. Control AIDS outbreak one of the leading indicators of the epidemic.