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应用ＰＣＲ技术检测２７０例孕６～１２周妇女外周血及相应绒毛组织中的弓形虫ＤＮＡ，结果弓形虫ＤＮＡ阳性２６例，相应的绒毛组织中检出８例，垂直传播率为３０．７７％（８／２６）。同时用ＥＬＩＳＡ法检测血清中弓形虫循环抗原（ＣＡｇ）、ＩｇＭ和ＩｇＧ抗体，结果阳性率分别为３．７０％、７，０３％和１５．５％。ＩｇＭ和／或ＣＡｇ阳性者，外周血皆检出弓形虫ＤＮＡ。提示应用ＰＣＲ技术检测绒毛组织弓形虫ＤＮＡ结合ＥＬＩＳＡ进行筛选，可早期诊断先天性弓形虫感染，较之用羊水、胎儿血进行产前诊断时间提前８～１２周，有利于尽早治疗或中止妊娠。
Toxoplasma gondii DNA was detected in 270 cases of pregnant women with 6 ~ 12 weeks of gestation and corresponding villi by PCR technique. Toxoplasma gondii DNA was positive in 26 cases, of which 8 cases were detected in the corresponding villus tissue. The vertical transmission rate was 30.77% (8/26). Serum Toxoplasma gondii antigens (CAg), IgM and IgG antibodies were detected by ELISA. The positive rates were 3.70%, 7,03% and 15.5% respectively. Toxoplasma gondii DNA was detected in both IgM and / or CAg positive peripheral blood. Prompt PCR detection of Toxoplasma gondii DNA-binding ELISA screening for early diagnosis of Toxoplasma gondii infection, compared with amniotic fluid, fetal blood prenatal diagnosis of time 8 to 12 weeks earlier, is conducive to early treatment or termination of pregnancy.