一、应用单克隆抗体区分淋巴细胞亚群在细胞表面存在着种类繁多的抗原。如用细胞本身免疫动物,将会产生多种抗体,用这种抗血清检出每个细胞特有的抗原困难很多。单克隆抗体是将一个抗体产生细胞与骨髓瘤细胞培养株合制成杂交瘤,或使之感染EB 病毒,细胞转化后,无限制地增殖的细胞集团(克隆)所产生的抗体,仅与单一抗原决定簇反应。应用这种单克隆抗体能精确地分析淋巴细胞表面抗原,已查明了T 细胞和B 细胞所固有的抗原,并了解在辅助性、抑制性及杀伤性T First, the use of monoclonal antibodies to distinguish lymphocyte subpopulations in the cell surface there is a wide range of antigens. Immunization of animals with the cells themselves produces multiple antibodies, and using this antiserum, it is much more difficult to detect the unique antigens of each cell. Monoclonal antibodies are antibodies produced by a group of antibody-producing cells and myeloma cell strains that have been made into hybridomas or infected with Epstein-Barr virus. The resulting antibodies are produced by a group of cells (clones) that proliferate without restriction after being transformed, Antigenic determinant response. The use of this monoclonal antibody enables the accurate analysis of lymphocyte surface antigens, has identified the antigens that are inherent in T cells and B cells, and has been elucidated on the basis of both helper, suppressive and lethal T